A Microarray Study of Articular Cartilage in Relation to Obesity and Severity of Knee Osteoarthritis.

ABSTRACT:

Objective

To query the transcript-level changes in the medial and lateral tibial plateau cartilage in tandem with obesity in patients with end-stage osteoarthritis (OA).

Design

Cartilage was obtained from 23 patients (20 obese [body mass index > 30 kg/m²], 3 overweight [body mass index < 30 kg/m²]) at the time of total knee replacement. Cartilage integrity was assessed using Outerbridge scale, while radiographic changes were scored on preoperative X-rays using Kellgren-Lawrence (K-L) classification. RNA was probed for differentially expressed transcripts between medial and lateral compartments using Affymetrix Gene 2.0 ST Array and validated via real-time polymerase chain reaction. Gene ontology and pathway analyses were also queried.

Results

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SUBMITTER: Rai MF 

PROVIDER: S-EPMC7488940 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature