Highly conserved extracellular residues mediate interactions between pore-forming and regulatory subunits of the yeast Ca²⁺ channel related to the animal VGCC/NALCN family.

ABSTRACT: Yeasts and fungi generate Ca²⁺ signals in response to environmental stresses through Ca²⁺ channels essentially composed of Cch1 and Mid1. Cch1 is homologous to the pore-forming α₁ subunit of animal voltage-gated Ca²⁺ channels (VGCCs) and sodium leak channels nonselective (NALCNs), whereas Mid1 is a membrane-associated protein similar to the regulatory α₂/δ subunit of VGCCs and the regulatory subunit of NALCNs. Although the physiological roles of Cch1/Mid1 channels are known, their molecular regulation remains elusive, including subunit interactions regulating channel functionality. Herein, we identify amino acid residues involved in interactions between the pore-forming Cch1 subunit and the essential regulatory Mid1 subunit of Sacch

SUBMITTER: Hayashi T 

PROVIDER: S-EPMC7489899 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature