Quantitative propagation of assembled human Tau from Alzheimer's disease brain in microfluidic neuronal cultures.

ABSTRACT: Tau aggregation and hyperphosphorylation is a key neuropathological hallmark of Alzheimer's disease (AD), and the temporospatial spread of Tau observed during clinical manifestation suggests that Tau pathology may spread along the axonal network and propagate between synaptically connected neurons. Here, we have developed a cellular model that allows the study of human AD-derived Tau propagation from neuron to neuron using microfluidic devices. We show by using high-content imaging techniques and an in-house developed interactive computer program that human AD-derived Tau seeds rodent Tau that propagates trans-neuronally in a quantifiable manner in a microfluidic culture model. Moreover, we were able to convert this model to a medium-throughput format allowing the user to handle 16 two-cha

SUBMITTER: Katsikoudi A 

PROVIDER: S-EPMC7489902 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature