The staphylococcal biofilm protein Aap forms a tetrameric species as a necessary intermediate before amyloidogenesis.

ABSTRACT: The accumulation-associated protein (Aap) from Staphylococcus epidermidis is a biofilm-related protein that was found to be a critical factor for infection using a rat catheter model. The B-repeat superdomain of Aap, composed of 5-17 B-repeats, each containing a Zn²⁺-binding G5 and a spacer subdomain, is responsible for Zn²⁺-dependent assembly leading to accumulation of bacteria during biofilm formation. We previously demonstrated that a minimal B-repeat construct (Brpt1.5) forms an antiparallel dimer in the presence of 2-3 Zn²⁺ ions. More recently, we have reported the presence of functional amyloid-like fibrils composed of Aap within S. epidermidis biofilms and demonstrated that a biologically relevant construct containing five and a half B-

SUBMITTER: Yarawsky AE 

PROVIDER: S-EPMC7489908 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature