Auxin-transporting ABC transporters are defined by a conserved D/E-P motif regulated by a prolylisomerase.

ABSTRACT: The plant hormone auxin must be transported throughout plants in a cell-to-cell manner to affect its various physiological functions. ABCB transporters are critical for this polar auxin distribution, but the regulatory mechanisms controlling their function is not fully understood. The auxin transport activity of ABCB1 was suggested to be regulated by a physical interaction with FKBP42/Twisted Dwarf1 (TWD1), a peptidylprolyl cis-trans isomerase (PPIase), but all attempts to demonstrate such a PPIase activity by TWD1 have failed so far. By using a structure-based approach, we identified several surface-exposed proline residues in the nucleotide binding domain and linker of Arabidopsis ABCB1, mutations of which do not alter ABCB1 protein stability or location but do affect its transpor

SUBMITTER: Hao P 

PROVIDER: S-EPMC7489919 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature