Chromatin Targeting of HIPK2 Leads to Acetylation-Dependent Chromatin Decondensation.

ABSTRACT: The protein kinase homeodomain-interacting protein kinase 2 (HIPK2) plays an important role in development and in the response to external cues. The kinase associates with an exceptionally large number of different transcription factors and chromatin regulatory proteins to direct distinct gene expression programs. In order to investigate the function of HIPK2 for chromatin compaction, HIPK2 was fused to the DNA-binding domains of Gal4 or LacI, thus allowing its specific targeting to binding sites for these transcription factors that were integrated in specific chromosome loci. Tethering of HIPK2 resulted in strong decompaction of euchromatic and heterochromatic areas. HIPK2-mediated heterochromatin decondensation started already 4 h after its chromatin association and required the function

SUBMITTER: Haas J 

PROVIDER: S-EPMC7490299 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature