Effects of perfluoroalkyl carboxylic acids on the uptake of sulfobromophthalein via organic anion transporting polypeptides in human intestinal Caco-2 cells.

ABSTRACT: We performed a detailed investigation of the uptake of sulfobromophthalein (BSP) from the apical membrane of Caco-2 cells, which is a substrate for organic anion transporting polypeptides (OATPs), and calculated the kinetic parameters of BSP uptake as follows: K_m = 13.9 ± 1.3 μM, V_max = 1.15 ± 0.07 nmol (mg protein)^-1 (5 min)^-1, and k_d = 38.2 ± 0.53 μL (mg protein)^-1 (5 min)^-1. Coincubation with medium-chain (C7-C11) perfluoroalkyl carboxylic acids (PFCAs), such as perfluoroheptanoic acid (PFHpA, C7), perfluorooctanoic acid (PFOA, C8), perfluorononanoic acid (PFNA, C9), perfluorodecanoic acid (PFDA, C10) and perfluoroundecanoic acid (PFUnDA, C11), significantly decreased BSP uptake by 27-55%, while coincubation wi

SUBMITTER: Kimura O 

PROVIDER: S-EPMC7490525 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature