Validation of reference genes for expression analysis in a murine trauma model combining traumatic brain injury and femoral fracture.

ABSTRACT: Systemic and local posttraumatic responses are often monitored on mRNA expression level using quantitative real-time PCR (qRT-PCR), which requires normalisation to adjust for confounding sources of variability. Normalisation requests reference (housekeeping) genes stable throughout time and divergent experimental conditions in the tissue of interest, which are crucial for a reliable and reproducible gene expression analysis. Although previous animal studies analysed reference genes following isolated trauma, this multiple-trauma gene expression analysis provides a notable study analysing reference genes in primarily affected (i.e. bone/fracture callus and hypothalamus) and secondarily affected organs (i.e. white adipose tissue, liver, muscle and spleen), following experimental long bone fr

SUBMITTER: Otto E 

PROVIDER: S-EPMC7490670 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature