Proton Transfer Reactions for the Gas-Phase Separation, Concentration, and Identification of Cardiolipins.

ABSTRACT: Cardiolipin (CL) analysis demands high specificity, due to the extensive diversity of CL structures, and high sensitivity, due to their low relative abundance within the lipidome. While electrospray ionization mass spectrometry (ESI-MS) is the most widely used technology in lipidomics, the potential for multiple charging presents unique challenges for CL identification and quantification. Depending on the conditions, ESI-MS of lipid extracts in negative ion mode can give rise to cardiolipins ionized as both singly and doubly deprotonated anions. This signal degeneracy diminishes the signal-to-noise ratio, while in addition (for direct infusion), the dianion population falls within a m/z range already heavily congested with monoanions from more abundant glycerophospholipid sub

SUBMITTER: Randolph CE 

PROVIDER: S-EPMC7490759 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature