Yan-Hou-Qing formula attenuates ammonia-induced acute pharyngitis in rats via inhibition of NF-κB and COX-2.

ABSTRACT:

Background

Yan Hou Qing (YHQ) is a Chinese medicinal formula designed to alleviate sore throat symptoms, but underlying mechanism of YHQ treatment for pharyngitis is poorly defined up to now.

Methods

In this study, the modulation of YHQ on pharyngitis is investigated in ammonia-induced acute pharyngitis rat models. After treatment with YHQ or dexamethasone respectively for five consecutive days, all rats were sacrificed for biomolecular and histopathologic study. Protein expressions of MAPKs, NF-κB, COX-2 and 5-LOX in pharyngitis tissue were evaluated by western blot analysis and the levels of TNF-α, IL-6, prostaglandin (PG) E₂, leukotrienes (LT)-B₄ and LT-D₄ in pharyngeal tissue were measured via ELISA assay. Evans blue (EB) dye exudation te

SUBMITTER: Xu M 

PROVIDER: S-EPMC7491126 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature