Development and clinical application of a novel CRISPR-Cas12a based assay for the detection of African swine fever virus.

ABSTRACT:

Background

As no treatment or effective vaccine for African swine fever virus (ASFV) is currently available, a rapid, highly sensitive diagnostic is urgently needed to curb the spread of ASFV.

Results

Here we designed a novel CRISPR-Cas12a based assay for ASFV detection. To detect different ASFV genotypes, 19 crRNAs were designed to target the conserved p72 gene in ASFV, and several crRNAs with high activity were identified that could be used as alternatives in the event of new ASFV variants. The results showed that the sensitivity of the CRISPR-Cas12a based assay is about ten times higher than either the commercial quantitative PCR (qPCR) kit or the OIE-recommended qPCR. CRISPR-Cas12a based assay could also detect ASFV specifically without cross-reactivity with other import

SUBMITTER: Wang X 

PROVIDER: S-EPMC7491166 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature