Structure elucidation of the redox cofactor mycofactocin reveals oligo-glycosylation by MftF.

ABSTRACT: Mycofactocin (MFT) is a redox cofactor belonging to the family of ribosomally synthesized and post-translationally modified peptides (RiPPs) and is involved in alcohol metabolism of mycobacteria including Mycobacterium tuberculosis. A preliminary biosynthetic model had been established by bioinformatics and in vitro studies, while the structure of natural MFT and key biosynthetic steps remained elusive. Here, we report the discovery of glycosylated MFT by ¹³C-labeling metabolomics and establish a model of its biosynthesis in Mycolicibacterium smegmatis. Extensive structure elucidation including NMR revealed that MFT is decorated with up to nine β-1,4-linked glucose residues including 2-O-methylglucose. Dissection of biosynthetic genes demonstrated tha

SUBMITTER: Pena-Ortiz L 

PROVIDER: S-EPMC7491314 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature