Phagocytic glioblastoma-associated microglia and macrophages populate invading pseudopalisades.

ABSTRACT: Hypoxic pseudopalisades are a pathological hallmark of human glioblastoma, which is linked to tumour malignancy and aggressiveness. Yet, their function and role in the tumour development have scarcely been explored. It is thought that pseudopalisades are formed by malignant cells escaping from the hypoxic environment, although evidence of the immune component of pseudopalisades has been elusive. In the present work, we analyse the immunological constituent of hypoxic pseudopalisades using high-resolution three-dimensional confocal imaging in tissue blocks from excised tumours of glioblastoma patients and mimic the hypoxic gradient in microfluidic platforms in vitro to understand the cellular motility. We visualize that glioblastoma-associated microglia and macrophages abundantly pop

SUBMITTER: Saavedra-Lopez E 

PROVIDER: S-EPMC7491442 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature