Complement opsonization of HIV affects primary infection of human colorectal mucosa and subsequent activation of T cells.

ABSTRACT: HIV transmission via genital and colorectal mucosa are the most common routes of dissemination. Here, we explored the effects of free and complement-opsonized HIV on colorectal tissue. Initially, there was higher antiviral responses in the free HIV compared to complement-opsonized virus. The mucosal transcriptional response at 24 hr revealed the involvement of activated T cells, which was mirrored in cellular responses observed at 96 hr in isolated mucosal T cells. Further, HIV exposure led to skewing of T cell phenotypes predominantly to inflammatory CD4+ T cells, that is Th17 and Th1Th17 subsets. Of note, HIV exposure created an environment that altered the CD8+ T cell phenotype, for example expression of regulatory factors, especially when the virions were opsonized with complement fact

SUBMITTER: Bhattacharya P 

PROVIDER: S-EPMC7492089 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature