Subcortical maternal complex (SCMC) expression during folliculogenesis is affected by oocyte donor age in sheep.

ABSTRACT:

Purpose

The age-associated decline in female fertility is largely ascribable to the decrease in oocyte quality. The subcortical maternal complex (SCMC) is a multiprotein complex essential for early embryogenesis and female fertility and functionally conserved across mammals. The present work evaluated expression dynamics of its components during folliculogenesis in relation to maternal age in sheep.

Methods

The expression of the SCMC components (KHDC3/FILIA, NLRP2, NLRP5/MATER, OOEP/FLOPED, PADI6, TLE6 and ZBED3) was analyzed by real-time PCR in pools of growing oocytes (GO) of different diameters (70-90 μm (S), 90-110 μm (M), or 110-130 μm (L)) derived from non-hormonally treated adult (Ad; age < 4 years), prepubertal (Pr; age 40 days), or aged ewes (age > 6 years).

Resu

SUBMITTER: Bebbere D 

PROVIDER: S-EPMC7492316 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature