Culture of human ovarian tissue in xeno-free conditions using laminin components of the human ovarian extracellular matrix.

ABSTRACT:

Purpose

Our purpose was to identify human ovarian extracellular matrix (ECM) components that would support in vitro culture of human ovarian tissue and be compatible with possible future clinical applications. We characterized ovarian expression of laminins and selected three laminin tripeptides for culture experiments to be compared with Matrigel, an undefined and animal-based mixture of ECM components.

Methods

Expression of the 12 laminin genes was determined on transcript and protein levels using cortical tissue samples (n = 6), commercial ovary RNA (n = 1), follicular fluid granulosa cells (n = 20), and single-cell RNA-sequencing data. Laminin 221 (LN221), LN521, LN511, and their mixture were chosen for a 7-day culture experiment along with Matrigel using tissue from 17

SUBMITTER: Hao J 

PROVIDER: S-EPMC7492356 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature