Omeprazole inhibits IgE-mediated mast cell activation and allergic inflammation induced by ingested allergen in mice.

ABSTRACT:

Background

Patients with eosinophilic esophagitis have increased numbers of mucosal mast cells. Administration of the proton pump inhibitor omeprazole can reduce both esophageal mast cell and eosinophil numbers and attenuate type 2 inflammation in these subjects.

Objective

Given that maintenance of an acidic environment within granules is important for mast cell homeostasis, we sought to evaluate the effects of omeprazole on mast cell functions including development, IgE:FcεRI-mediated activation, and responses to food allergen.

Methods

Mast cell degranulation, cytokine secretion, and early signaling events in the FcεRI pathway, including protein kinase phosphorylation and Ca²⁺ flux, were measured after IgE crosslinking in murine bone marrow-derived mast ce

SUBMITTER: Kanagaratham C 

PROVIDER: S-EPMC7492424 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature