PARK7 Diminishes Oxidative Stress-Induced Mucosal Damage in Celiac Disease.

ABSTRACT: Coeliac disease (CD) is a chronic, immune-mediated small intestinal enteropathy, accompanied with gluten-triggered oxidative damage of duodenal mucosa. Previously, our research group reported an increased mucosal level of the antioxidant protein Parkinson's disease 7 (PARK7) in children with CD. In the present study, we investigated the role of increased PARK7 level on the epithelial cell and mucosal integrity of the small intestine. The presence of PARK7 was investigated using immunofluorescent staining on duodenal mucosa of children with CD and on FHs74Int duodenal epithelial cells. To investigate the role of oxidative stress, FHs74Int cells were treated with H₂O₂ in the absence or presence of Comp23, a PARK7-binding compound. Intracellular accumulation of reactive

SUBMITTER: Veres-Szekely A 

PROVIDER: S-EPMC7492931 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature