A stoichiometric and pseudo kinetic model of loop mediated isothermal amplification.

ABSTRACT: Loop mediated isothermal amplification (LAMP) is one of the most popular isothermal DNA amplification techniques for research and commercial applications, enabling amplification of both DNA and RNA (with the assistance of reverse transcriptase). The LAMP mechanism is powered by strategic primer design and a strand displacement polymerase, generating products that fold over, creating loops. LAMP leads to generation of products of increasing length over time. These products containing multiple loops are conventionally called cauliflower structures. Existing literature on LAMP provides extremely limited understanding of progression of cascades of reactions involved in the reaction and it is believed that cauliflower structures of increasing length constitute a majority of the product formed i

SUBMITTER: Kaur N 

PROVIDER: S-EPMC7493047 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature