Reagent-Free Colorimetric Cholesterol Test Strip Based on Self Color-Changing Property of Nanoceria.

ABSTRACT: Paper-based test strip consisting of cerium oxide nanoparticles (nanoceria) as hydrogen peroxide (H₂O₂)-dependent color-changing nanozymes and cholesterol oxidase (ChOx) has been developed for convenient colorimetric determination of cholesterol without the need for chromogenic substrate. The construction of the cholesterol strip begins with physical adsorption of nanoceria on the paper surface, followed by covalent immobilization of ChOx via silanization, chitosan-mediated activation, and glutaraldehyde treatment of the nanoceria-embedded paper matrices. In the presence of cholesterol, ChOx catalyzes its oxidation to produce H₂O₂, which forms peroxide complex on the nanoceria surface and induces visual color change of the nanoceria-embedded pape

SUBMITTER: Nguyen PT 

PROVIDER: S-EPMC7493407 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature