Value of swab types and collection time on SARS-COV-2 detection using RT-PCR assay.

ABSTRACT:

Objective

Low viral load from patients infected with SARS-CoV-2 during infection late stage easily lead to false negative nucleic acid testing results, thus having great challenges to the prevention and control of the current pandemic. In present study, we mainly aimed to evaluate specimen types and specimen collection timepoint on the positive detection of 2019 novel coronavirus from patients at infection late stage based on RT-PCR testing.

Methods

Paired nasopharyngeal swabs, nasal swabs, oropharyngeal swabs and anal swabs were collected from patients infected with SARS-CoV-2 during infection late stage before washing in the morning and afternoon on the same day. Then virus RNA was extracted and tested for 2019-nCoV identification by RT-PCR within 24 h.

Results

Vira

SUBMITTER: Liu M 

PROVIDER: S-EPMC7493793 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature