Ontology highlight
ABSTRACT:
SUBMITTER: Mao D
PROVIDER: S-EPMC7493819 | biostudies-literature | 2020 Jul
REPOSITORIES: biostudies-literature
Mao Dainan D Yoshimura Aya A Wang Rurun R Seyedsayamdost Mohammad R MR
Chembiochem : a European journal of chemical biology 20200303 13
Most natural product biosynthetic gene clusters that can be observed bioinformatically are silent. This insight has prompted the development of several methodologies for inducing their expression. One of the more recent methods, termed reporter-guided mutant selection (RGMS), entails creation of a library of mutants that is then screened for the desired phenotype via reporter gene expression. Herein, we apply a similar approach to Burkholderia thailandensis and, using transposon mutagenesis, mut ...[more]