Long-read sequencing and de novo genome assembly of marine medaka (Oryzias melastigma).

ABSTRACT:

Background

Marine medaka (Oryzias melastigma) is considered as an important ecotoxicological indicator to study the biochemical, physiological and molecular responses of marine organisms towards increasing amount of pollutants in marine and estuarine waters.

Results

In this study, we reported a high-quality and accurate de novo genome assembly of marine medaka through the integration of single-molecule sequencing, Illumina paired-end sequencing, and 10X Genomics linked-reads. The 844.17 Mb assembly is estimated to cover more than 98% of the genome and is more continuous with fewer gaps and errors than the previous genome assembly. Comparison of O. melastigma with closely related species showed significant expansion of gene families associated with DNA repair and ATP-binding

SUBMITTER: Liang P 

PROVIDER: S-EPMC7493909 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature