Advantage of fat-derived CD73 positive cells from multiple human tissues, prospective isolated mesenchymal stromal cells.

ABSTRACT: Somatic stem cells have been isolated from multiple human tissues for their potential usefulness in cell therapy. Currently, mesenchymal stromal cells (MSCs) are prepared after several passages requiring a few months of cell culture. In this study, we used a prospective isolation method of somatic stem cells from gestational or fat tissues, which were identified using CD73 antibody. CD73-positive population from various tissues existed individually in flowcytometric pattern, especially subcutaneous fat- and amniotic-derived cells showed the highest enrichment of CD73-positive cells. Moreover, the cell populations isolated with the prospective method showed higher proliferative capacity and stem cell marker expression, compared to the cell populations which isolated through several passages

SUBMITTER: Suto EG 

PROVIDER: S-EPMC7493914 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature