EGFR/Ras-induced CCL20 production modulates the tumour microenvironment.

ABSTRACT:

Background

The activation of the EGFR/Ras-signalling pathway in tumour cells induces a distinct chemokine repertoire, which in turn modulates the tumour microenvironment.

Methods

The effects of EGFR/Ras on the expression and translation of CCL20 were analysed in a large set of epithelial cancer cell lines and tumour tissues by RT-qPCR and ELISA in vitro. CCL20 production was verified by immunohistochemistry in different tumour tissues and correlated with clinical data. The effects of CCL20 on endothelial cell migration and tumour-associated vascularisation were comprehensively analysed with chemotaxis assays in vitro and in CCR6-deficient mice in vivo.

Results

Tumours facilitate progression by the EGFR/Ras-induced production of CCL20. Expression of the chemokine CCL20

SUBMITTER: Hippe A 

PROVIDER: S-EPMC7493992 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature