Gradient diffusion susceptibility testing for Neisseria gonorrhoeae: an accurate alternative to agar dilution in high-MIC strains?

ABSTRACT:

Introduction

The correlation of antimicrobial susceptibility testing (AST) between agar dilution and gradient diffusion for Neisseria gonorrhoeae is not well established, especially in strains with high MICs.

Aim

The objective of this study was to evaluate the accuracy of gradient diffusion for N. gonorrhoeae .

Methods

Fifty strains of N. gonorrhoeae , all tested by the agar dilution method according to CLSI methods and confirmed to be genetically distinct using molecular typing (NG-MAST), were selected. Isolates with high MICs were targeted. Gradient diffusion was performed for ceftriaxone (CRO), cefixime (CFX), azithromycin (AZT), tetracycline (TET) and fosfomycin (FOS) using two different commercial antimicrobial strips on different culture med

SUBMITTER: Desjardins M 

PROVIDER: S-EPMC7494190 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature