Stabilizer Cell Gene Therapy: A Less-Is-More Strategy to Prevent Cardiac Arrhythmias.

ABSTRACT:

Background

In cardiac gene therapy to improve contractile function, achieving gene expression in the majority of cardiac myocytes is essential. In preventing cardiac arrhythmias, however, this goal may not be as important since transduction efficiencies as low as 40% suppressed ventricular arrhythmias in genetically modified mice with catecholaminergic polymorphic ventricular tachycardia.

Methods

Using computational modeling, we simulated 1-, 2-, and 3-dimensional tissue under a variety of conditions to test the ability of genetically engineered nonarrhythmogenic stabilizer cells to suppress triggered activity due to delayed or early afterdepolarizations.

Results

Due to source-sink relationships in cardiac tissue, a minority (20%-50%) of randomly distributed stabilize

SUBMITTER: Liu MB 

PROVIDER: S-EPMC7494545 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature