Aging Disrupts Normal Time-of-Day Variation in Cardiac Electrophysiology.

ABSTRACT:

Background

Cardiac gene expression and arrhythmia occurrence have time-of-day variation; however, daily changes in cardiac electrophysiology, arrhythmia susceptibility, and Ca²⁺ handling have not been characterized. Furthermore, how these patterns change with age is unknown.

Methods

Hearts were isolated during the light (zeitgeber time [ZT] 4 and ZT9) and dark cycle (ZT14 and ZT21) from adult (12-18 weeks) male mice. Hearts from aged (18-20 months) male mice were isolated at ZT4 and ZT14. All hearts were Langendorff-perfused for optical mapping with voltage- and Ca²⁺-sensitive dyes (n=4-7/group). Cardiac gene and protein expression were assessed with real-time polymerase chain reaction (n=4-6/group) and Western blot (n=3-4/group).

Results

Adult h

SUBMITTER: Wang Z 

PROVIDER: S-EPMC7494582 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature