Dataset Information

Redefining the Clostridioides difficile σ^B Regulon: σ^B Activates Genes Involved in Detoxifying Radicals That Can Result from the Exposure to Antimicrobials and Hydrogen Peroxide.

ABSTRACT: In many Gram-positive bacteria, the general stress response is regulated at the transcriptional level by the alternative sigma factor sigma B (σ^B). In C. difficile, σ^B has been implicated in protection against stressors such as reactive oxygen species (ROS) and antimicrobial compounds. Here, we used an anti-σ^B antibody to demonstrate time-limited overproduction of σ^B in C. difficile despite its toxicity at higher cellular concentrations. This toxicity eventually led to the loss of the plasmid used for anhydrotetracycline-induced σ^B gene expression. Inducible σ^B overproduction uncouples σ^B expression from its native regulatory network and allows for the refinement of the previously proposed σ^B

SUBMITTER: Boekhoud IM

PROVIDER: S-EPMC7494833 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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Dataset Information

Redefining the Clostridioides difficile σ^B Regulon: σ^B Activates Genes Involved in Detoxifying Radicals That Can Result from the Exposure to Antimicrobials and Hydrogen Peroxide.

OmicsDI is part of the ELIXIR infrastructure

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Dataset Information

Redefining the Clostridioides difficile σB Regulon: σB Activates Genes Involved in Detoxifying Radicals That Can Result from the Exposure to Antimicrobials and Hydrogen Peroxide.

OmicsDI is part of the ELIXIR infrastructure

Tweets

Redefining the Clostridioides difficile σ^B Regulon: σ^B Activates Genes Involved in Detoxifying Radicals That Can Result from the Exposure to Antimicrobials and Hydrogen Peroxide.