Lung immunoglobulin A immunity dysregulation in cystic fibrosis.

ABSTRACT:

Background

In cystic fibrosis (CF), recurrent infections suggest impaired mucosal immunity but whether production of secretory immunoglobulin A (S-IgA) is impaired remains elusive. S-IgA is generated following polymeric immunoglobulin receptor (pIgR)-mediated transepithelial transport of dimeric (d-)IgA and represents a major defence through neutralisation of inhaled pathogens like Pseudomonas aeruginosa (Pa).

Methods

Human lung tissue (n = 74), human sputum (n = 118), primary human bronchial epithelial cells (HBEC) (cultured in air-liquid interface) (n = 19) and mouse lung tissue and bronchoalveolar lavage were studied for pIgR expression, IgA secretion and regulation.

Findings

Increased epithelial pIgR immunostaining was observed in CF lung explants, associated with

SUBMITTER: Collin AM 

PROVIDER: S-EPMC7495088 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature