Smad3 signalling affects high glucose-induced podocyte injury via regulation of the cytoskeletal protein transgelin.

ABSTRACT:

Aim

The aim of the present study was to characterize the role of Smad3 signalling on high glucose-induced podocyte injury.

Methods

Synchronized conditionally immortalized mouse podocyte cell line (MPC5) cells were treated with either D-glucose alone or D-glucose plus the Smad3 inhibitor SIS3. The distribution of F-actin and transgelin in a high glucose-induced model of podocyte injury were examined by immunofluorescence. Levels of transgelin and Smad3 signalling proteins in MPC5 cells were determined by Western blot.

Results

A disordered distribution of F-actin, as well as co-localization of F-actin and transgelin, was observed in podocytes exposed to high glucose. Increased levels of transgelin were first observed 10 minutes after treatment with glucose, suggesting t

SUBMITTER: Jiang L 

PROVIDER: S-EPMC7496067 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature