Xdrop: Targeted sequencing of long DNA molecules from low input samples using droplet sorting.

ABSTRACT: Long-read sequencing can resolve regions of the genome that are inaccessible to short reads, and therefore are ideal for genome-gap closure, solving structural rearrangements and sequencing through repetitive elements. Here we introduce the Xdrop technology: a novel microfluidic-based system that allows for targeted enrichment of long DNA molecules starting from only a few nanograms of DNA. Xdrop is based on the isolation of long DNA fragments in millions of droplets, where the droplets containing a target sequence of interest are fluorescently labeled and sorted using flow cytometry. The final product from the Xdrop procedure is an enriched population of long DNA molecules that can be investigated by sequencing. To demonstrate the capability of Xdrop, we performed enrichment of the human

SUBMITTER: Madsen EB 

PROVIDER: S-EPMC7496172 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature