Quick microbial molecular phenotyping by differential shotgun proteomics.

ABSTRACT: Differential shotgun proteomics identifies proteins that discriminate between sets of samples based on differences in abundance. This methodology can be easily applied to study (i) specific microorganisms subjected to a variety of growth or stress conditions or (ii) different microorganisms sampled in the same condition. In microbiology, this comparison is particularly successful because differing microorganism phenotypes are explained by clearly altered abundances of key protein players. The extensive description and quantification of proteins from any given microorganism can be routinely obtained for several conditions within a few days by tandem mass spectrometry. Such protein-centred microbial molecular phenotyping is rich in information. However, well-designed experimental strategies,

SUBMITTER: Gouveia D 

PROVIDER: S-EPMC7496289 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature