Immunological dynamics after subcutaneous immunization with a squalene-based oil-in-water adjuvant.

ABSTRACT: The clinically successful adjuvant MF59 is used in seasonal influenza vaccines, which is proposed to enhance immunity by creating an immune-competent microenvironment in the muscle that allows recruitment of immune cells that drive adaptive immune responses. Here, we examined whether the clinically successful adjuvants MF59/AddaVax could be used for subcutaneous use and how antigen delivery can be synergized with cellular dynamics at the vaccination site. Subcutaneous injection of AddaVax leads to thickening of the skin, characterized by a neutrophil-monocyte recruitment sequence. Skin-infiltrating CCR2⁺Ly6C^high monocytes showed differentiation to CD11b⁺Ly6C⁺MHCII⁺CD11c⁺CD64⁺ monocyte-derived DCs over time in the

SUBMITTER: Schetters STT 

PROVIDER: S-EPMC7496326 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature