In-vitro spermatogenesis through testis modelling: Toward the generation of testicular organoids.

ABSTRACT:

Background

The testicular organoid concept has recently been introduced in tissue engineering to refer to testicular cell organizations modeling testicular architecture and function. The testicular organoid approach gives control over which and how cells reaggregate, which is not possible in organotypic cultures, thereby extending the applicability of in-vitro spermatogenesis (IVS) systems. However, it remains unclear which culture method and medium allow reassociation of testicular cells into a functional testicular surrogate in-vitro.

Objective

The aim of this paper is to review the different strategies that have been used in an attempt to create testicular organoids and generate spermatozoa. We want to provide an up-to-date list on culture methodologies and media composit

SUBMITTER: Richer G 

PROVIDER: S-EPMC7496450 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature