The role of reactive oxygen species in the virulence of wheat leaf rust fungus Puccinia triticina.

ABSTRACT: Reactive oxygen species (ROS) play an important role during host-pathogen interactions and are often an indication of induced host defence responses. In this study, we demonstrate for the first time that Puccinia triticina (Pt) generates ROS, including superoxide, H₂ O₂ and hydroxyl radicals, during wheat infection. Through pharmacological inhibition, we found that ROS are critical for both Pt urediniospore germination and pathogenic development on wheat. A comparative RNA-Seq analysis of different stages of Pt infection process revealed 291 putative Pt genes associated with the oxidation-reduction process. Thirty-seven of these genes encode known proteins. The expressions of five Pt genes, including PtNoxA, PtNoxB, PtNoxR, PtCat and PtSod, were subsequently verified

SUBMITTER: Wang X 

PROVIDER: S-EPMC7496513 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature