Comprehensive characterization of myeloid cells during wound healing in healthy and healing-impaired diabetic mice.

ABSTRACT: Wound healing involves the concerted action of various lymphoid and in particular myeloid cell populations. To characterize and quantitate different types of myeloid cells and to obtain information on their kinetics during wound healing, we performed multiparametric flow cytometry analysis. In healthy mice, neutrophil numbers increased early after injury and returned to near basal levels after completion of healing. Macrophages, monocyte-derived dendritic cells (DCs), and eosinophils were abundant throughout the healing phase, in particular in early wounds, and Langerhans cells increased after wounding and remained elevated after epithelial closure. Major differences in healing-impaired diabetic mice were a much higher percentage of immune cells in late wounds, mainly as a result of neutro

SUBMITTER: Joshi N 

PROVIDER: S-EPMC7496577 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature