Inhibition of miR-103a-3p suppresses lipopolysaccharide-induced sepsis and liver injury by regulating FBXW7 expression.

ABSTRACT: Inflammation, apoptosis, and oxidative stress are involved in septic liver dysfunction. Herein, the role of miR-103a-3p/FBXW7 axis in lipopolysaccharides (LPS)-induced septic liver injury was investigated in mice. Hematoxylin-eosin staining was used to evaluate LPS-induced liver injury. Quantitative real-time polymerase chain reaction was performed to determine the expression of microRNA (miR) and messenger RNA, and western blot analysis was conducted to examine the protein levels. Dual-luciferase reporter assay was used to confirm the binding between miR-103a-3p and FBXW7. Both annexin V-fluoresceine isothiocyanate/propidium iodide staining and caspase-3 activity were employed to determine cell apoptosis. First, miR-103a-3p was upregulated in the septic serum of mice and patients with sep

SUBMITTER: Zhou YP 

PROVIDER: S-EPMC7496651 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature