Ratiometric Detection of ATP by Fluorescent Cyclophanes with Bellows-Type Sensing Mechanism.

ABSTRACT: Pyrene-based cyclophanes have been synthesized with the aim to realize a bellows-type sensing mechanism for the ratiometric detection of nucleotide concentrations in a buffered aqueous solution. The sensing mechanism involves the encapsulation of a nucleobase between two pyrene rings, which affects the monomer-excimer equilibrium of the receptor in the excited state. The nature of the spacer and its connection pattern to pyrene rings have been varied to achieve high selectivity for ATP. The 1,8-substituted pyrene-based cyclophane with the 2,2'-diaminodiethylamine spacer demonstrates the best selectivity for ATP showing a 50-fold increase in the monomer-excimer emission ratio upon saturation with the nucleotide. The receptor can detect ATP within the biological concentrations range over a w

SUBMITTER: Agafontsev AM 

PROVIDER: S-EPMC7496914 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature