A linked-read approach to museomics: Higher quality de novo genome assemblies from degraded tissues.

ABSTRACT: High-throughput sequencing technologies are a proposed solution for accessing the molecular data in historical specimens. However, degraded DNA combined with the computational demands of short-read assemblies has posed significant laboratory and bioinformatics challenges for de novo genome assembly. Linked-read or "synthetic long-read" sequencing technologies, such as 10× Genomics, may provide a cost-effective alternative solution to assemble higher quality de novo genomes from degraded tissue samples. Here, we compare assembly quality (e.g., genome contiguity and completeness, presence of orthogroups) between four new deer mouse (Peromyscus spp.) genomes assembled using linked-read technology and four published genomes assembled from a single shotgun library. At a similar price-point, the

SUBMITTER: Colella JP 

PROVIDER: S-EPMC7496956 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature