Preoperative evaluation of microvascular invasion with circulating tumour DNA in operable hepatocellular carcinoma.

ABSTRACT:

Background & aims

Microvascular invasion (MVI) is a critical prognostic factor for operable hepatocellular carcinoma (HCC). This study aimed to explore the performance of circulating tumour DNA (ctDNA) in evaluating MVI status preoperatively.

Methods

Seventy-three HCC patients were enrolled and randomly divided into a training cohort and a validation cohort in a 2:1 ratio, and preoperative blood and surgical tissue samples were obtained. Genomic alterations were analysed using targeted deep sequencing with a 1021-gene panel.

Results

In training cohort, 260 somatic mutations were identified in 40 blood samples (81.6%). CtDNA mutation was verified in paired tissue sample in 39 patients (97.5%). In univariate analysis, ctDNA allele frequency (AF) and largest tumour diame

SUBMITTER: Wang D 

PROVIDER: S-EPMC7496978 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature