Antimicrobial solid media for screening non-sterile Arabidopsis thaliana seeds.

ABSTRACT: Stable genetic transformation of plants is a low-efficiency process, and identification of positive transformants usually relies on screening for expression of a co-transformed marker gene. Often this involves germinating seeds on solid media containing a selection reagent. Germination on solid media requires surface sterilization of seeds and careful aseptic technique to prevent microbial contamination, but surface sterilization techniques are time consuming and can cause seed mortality if not performed carefully. We developed an antimicrobial cocktail that can be added to solid media to inhibit bacterial and fungal growth without impairing germination, allowing us to bypass the surface sterilization step. Adding a combination of terbinafine (1 μM) and timentin (200 mg l-1 ) to Murashige

SUBMITTER: Behrendorff JBYH 

PROVIDER: S-EPMC7497060 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature