Mushroom DNA barcoding project: Sequencing a segment of the 28S rRNA gene.

ABSTRACT: DNA barcoding is an important molecular methodology for species identification that was developed over the last two decades and it should be covered in the biology bachelor curriculum. Here, we present an example of DNA barcoding by sequencing a segment of the 28S nuclear ribosomal large subunit rRNA gene of wild mushrooms and framing the education in a project form for undergraduate students in biology. Students perform this project in 6-8 weeks, which also includes preparing a poster, writing a report and presenting a paper related to the work in a journal club format. First, fieldwork in the Netherlands was carried out, during which students collected mushrooms under supervision of a professional mycologist with the goal to (a) verify morphologically based identifications with a molecul

SUBMITTER: Horn IR 

PROVIDER: S-EPMC7497104 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature