Diagnostic implications of mycetoma derived from Madurella pseudomycetomatis isolates from Mexico.

ABSTRACT:

Background

At the dermatology service of the General Hospital of Mexico City, Mexico, two patients, father and son, with black-grain mycetoma were seen. The grains were isolated, and the cultured fungi were identified as Madurella mycetomatis based on morphology. Using the M. mycetomatis specific PCR, amplicons of a different size than that of the M. mycetomatis type strain were obtained.

Objective

To determine the causative agent of the two black-grain mycetoma cases and develop non-culture-based diagnostic tools to identify them to the species level.

Methods

The M. mycetomatis specific, the internal transcribed spacer (ITS) region, β-tubulin (BT) and ribosomal binding protein 2 (RBP2) PCRs were used to confirm the identity of the isolates. Genetic variation was esta

SUBMITTER: Nyuykonge B 

PROVIDER: S-EPMC7497165 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature