Embryonic disc formation following post-hatching bovine embryo development in vitro.

ABSTRACT: Failures during conceptus elongation are a major cause of pregnancy losses in ungulates, exerting a relevant economic impact on farming. The developmental events occurring during this period are poorly understood, mainly because this process cannot be recapitulated in vitro. Previous studies have established an in vitro post-hatching development (PHD) system that supports bovine embryo development beyond the blastocyst stage, based on agarose gel tunnels and serum- and glucose-enriched medium. Unfortunately, under this system embryonic disc formation is not achieved and embryos show notorious signs of apoptosis and necrosis. The objective of this study has been to develop an in vitro system able to support embryonic disc formation. We first compared post-hatching development inside agarose

SUBMITTER: Ramos-Ibeas P 

PROVIDER: S-EPMC7497357 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature