Point-of-care DNA testing by automatically and sequentially performing extraction, amplification and identification in a closed-type cassette.

ABSTRACT: Nucleic acid detection is important for clinical diagnostics; however, it is challenging to perform genetic testing at the point-of-care due to the tedious steps involved in DNA extraction and the risk of cross-contamination from amplicons. To achieve a fully-automated and contamination-free nucleic acid detection, we propose a closed-type cassette system which enables the following steps to be operated automatically and sequentially: sample preparation based on magnetic beads, target amplification using multiplex polymerase chain reaction, and colorimetric detection of amplicons using a serial invasive reaction coupled with the aggregation of gold nanoparticle probes. The cassette was designed to be round and closed, and 10 targets in a sample could be simultaneously detected by the naked

SUBMITTER: Dong T 

PROVIDER: S-EPMC7497388 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature