Expression Profiles of CircRNA and mRNA in Lacrimal Glands of AQP5^-/- Mice With Primary Dry Eye.

ABSTRACT: Purpose: This work aimed to identify differentially expressed circular RNAs (circRNAs) and elucidate their potential function in aquaporin 5 (AQP5) knockout (AQP5^-/-) mice with the primary dry eye phenotype. Methods: A slit lamp examination was performed on AQP5^-/- mice to assess corneal epithelial defects using fluorescein sodium staining. Hematoxylin-eosin staining and transmission electron microscopy analysis were performed to identify structural changes in lacrimal gland epithelial cells due to AQP5 deficiency. The expression profiles of circRNA and messenger RNA (mRNA) were determined by a microarray analysis. The selected circRNA was verified by quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR). Gene Ontology and Kyot

SUBMITTER: Liu Y 

PROVIDER: S-EPMC7497440 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature