Development of a LC-MS/MS method using stable isotope dilution for the quantification of individual B₆ vitamers in fruits, vegetables, and cereals.

ABSTRACT: Vitamin B₆ comprises an important set of molecules tightly interwoven with the human amino acid, fatty acid, and carbohydrate metabolism. Analytical methods striving for the quantification of individual B₆ vitamers so far mostly rely on methods based on HPLC in combination with fluorescence detection, but their application encounters multiple difficulties due to the chemical divergence of the single vitamers. The present study describes the development of a method based on LC-MS/MS and stable isotope dilution assay (SIDA) for the simultaneous quantification of five vitamers (PN, PL, PM, PMP, and PNG) of the B₆ group in food samples. [¹³C₃]-PN, [¹³C₃]-PL, and [¹³C₆]-PNG were applied as interna

SUBMITTER: Bachmann T 

PROVIDER: S-EPMC7497486 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature