Loss of progesterone receptor is associated with distinct tyrosine kinase profiles in breast cancer.

ABSTRACT:

Purpose

The aim of this study was to assess protein tyrosine kinase profiles in primary breast cancer samples in correlation with the distinct hormone and growth receptor profiles ER, PR, and HER2.

Experimental design

Pamchip® microarrays were used to measure the phosphorylation of 144 tyrosine kinase substrates in 29 ER+ breast cancer samples and cell lines MCF7, BT474 and ZR75-1. mRNA expression data from the METABRIC cohort and publicly available PR chip-sequencing data were used for validation purposes, together with RT-PCR.

Results

In ER+ breast tumors and cell lines, we observed that the loss of PR expression correlated to higher kinase activity in samples and cell lines that were HER2-. A number of kinases, representing mostly proteins within the PI3K/AKT pathw

SUBMITTER: Tahiri A 

PROVIDER: S-EPMC7497693 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature