FANCF hypomethylation is associated with colorectal cancer in Han Chinese.

ABSTRACT:

Background/aims

Fanconi anemia complement group F (FANCF) is known to be involved in DNA repair, and the overexpression of FANCF protein leads to cell proliferation and ultimately to cancer. The purpose of this study was to assess whether FANCF methylation was associated with colorectal cancer (CRC).

Materials and methods

A case-control experiment was conducted to study the association between FANCF methylation and CRC. We used quantitative methylation-specific PCR to measure the FANCF promoter methylation, and the percentage of methylation reference (PMR) to quantify the FANCF promoter methylation level. To investigate the effect of the selected FANCF fragment on gene expression regulation, we also performed a dual-luciferase reporter gene assay.

Results

The results

SUBMITTER: Yu H 

PROVIDER: S-EPMC7498186 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature